Revista de la Sociedad Argentina de Diabetes (Nov 2020)

P31 Intergenerational transmission of metabolic alterations in the offspring of rats with diabetes

  • Daiana Fornes,
  • Florencia Heinecke,
  • Alicia Jawerbaum,
  • Evangelina Capobianco

DOI
https://doi.org/10.47196/diab.v54i3Sup.416
Journal volume & issue
Vol. 54, no. 3Sup
pp. 136 – 136

Abstract

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Introduction: The exposure to an adverse intrauterine environment, observed in the offspring of pregnancies complicated by diabetes, can program susceptibility to metabolic, endocrine and cardiovascular disorders later in life. Paternal exposure to diabetes can also lead to the development of metabolic disorders in the offspring, but this has been less studied. Objective: Our aim was to evaluate the alterations in the lipid metabolism of placentas from fetuses (F2) fathered by male rats with type 2 diabetes (F1), obtained by intrauterine programming of rats with moderate pregestational diabetes (F0). Methods: Control and type 2 diabetic male rats (diabetes obtained by intrauterine programming in the offspring of streptozotocin-induced diabetic rats, glycemia: 140-190 mg/dL) were mated with control female rats. On day 21 of gestation, the placenta of male and female fetuses was obtained for the evaluation of lipid levels (by TLC), mRNA of genes involved in lipid metabolism (by RT-qPCR) and lipoperoxidation (quantification of TBARS).

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