Derivation of the human induced pluripotent stem cell line MUi017-A from a patient with homozygous Hemoglobin Constant Spring

Wasinee Wongkummool; Warun Maneepitasut; Thongperm Munkongdee; Pirut Tong-ngam; Amornrat Tangprasittipap; Saovaros Svasti; Narisorn Kitiyanant; Kittiphong Paiboonsukwong; Suthat Fucharoen; Alisa Tubsuwan

doi:10.1016/j.scr.2017.02.014

Stem Cell Research (Apr 2017)

Derivation of the human induced pluripotent stem cell line MUi017-A from a patient with homozygous Hemoglobin Constant Spring

Wasinee Wongkummool,
Warun Maneepitasut,
Thongperm Munkongdee,
Pirut Tong-ngam,
Amornrat Tangprasittipap,
Saovaros Svasti,
Narisorn Kitiyanant,
Kittiphong Paiboonsukwong,
Suthat Fucharoen,
Alisa Tubsuwan

Affiliations

Wasinee Wongkummool: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Warun Maneepitasut: Molecular Medicine Program, Multidisciplinary Unit, Faculty of Science, Mahidol University, 10400, Thailand
Thongperm Munkongdee: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Pirut Tong-ngam: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Amornrat Tangprasittipap: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Saovaros Svasti: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Narisorn Kitiyanant: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Kittiphong Paiboonsukwong: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Suthat Fucharoen: Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand
Alisa Tubsuwan: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand

DOI: https://doi.org/10.1016/j.scr.2017.02.014
Journal volume & issue: Vol. 20, no. C
pp. 84 – 87

Abstract

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Hemoglobin Constant Spring (HbCS, HBA2: c.427T>C) is a common nondeletional α-thalassemia resulting from a nucleotide substitution at the termination codon of the HBA2 gene. Homozygosity for HbCS is characterized with mild anemia, jaundice, and splenomegaly. In this study, the human induced pluripotent stem cell line MUi017-A was successfully generated from peripheral blood CD34+ hematopoietic progenitors of a 52 year old female with homozygous HbCS. The MUi017-A cell line exhibited embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of differentiating into the three germ layers. The cell line may be used for the disease modeling.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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