Molecular identification of Haemonchus contortus in goats

Abstract

Haemonchus contortus commonly called the stomach worm or wire worm of ruminants inhabits the abomasum and is considered to be one of the economically important gastrointestinal strongyles in goats. In the present study, H. contortus was identified by PCR using the primers targeting partial 5.8S and partial internal transcribed spacer region 2 (ITS-2). Adult worms were identified morphologically and genomic DNA was extracted using DNeasy Blood and Tissue kit (QIAGEN, Germany). Gradient PCR protocol was standardised using the extracted genomic DNA. Ten-fold serial dilution of adult DNA was used to analyse the minimum detection limit and the products were amplified upto the tenth dilution. Cross reaction of primer sets was checked using the DNA extracted from predominant adult srongyles like Oesophagostomum columbianum and Trichostrongylus colubriformis and no cross reaction was seen at the optimum annealing temperature (60.7°C).

Keywords

• haemonchus contortus
• goats
• pcr
• its-2