Role of Ca2+, Calnexin and Calreticulin in Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura

Abstract

Da-Ming Xu,1,* Ze-Wen Zhang,2,* Jing-Xing Yi,3 Long Xie,3 Wen-Jun Yu,2 Jin-Feng Qiu,4 Cheng-Wei Xu,5 Chun-Ling He,6 Xian-Ru Xu,7 Jun Yin3 1Division of Urological Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 2Division of Hematology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 3Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 4Division of Respirology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 5Department of Blood Purification, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 6Department of Pathology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China; 7Division of Inventional Ultrasonic Therapeutics, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun Yin, Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Dongxia Road North, Shantou, Guangdong Province, 515041, People’s Republic of China, Tel [phone], Email [email protected]: Adult chronic immune thrombocytopenia (chronic ITP) is a common autoimmune hemorrhagic disease characterized by decreased platelet production and increased platelet destruction, leading to thrombocytopenia. In this study, Ca2+, calnexin (CNX) and calreticulin (CRT) within platelets from adult patients with chronic ITP were investigated.Methods: Platelets were isolated from blood specimen collected from 20 adult patients with chronic ITP and 20 healthy volunteers. Ca2+, CNX and CRT were determined by flow cytometry, and the results were analyzed with EXPO32 ADC software.Results: Flow cytometry showed the expressions of Ca2+ (74.19± 19.40% vs 22.79± 10.47%) was elevated (P< 0.05). However, CNX (15.10± 7.32% vs 41.79± 14.45%) and CRT (25.11± 12.66% vs 38.58± 12.02%) were decreased markedly in platelets from adult patients with chronic ITP (P< 0.05 compared with healthy volunteers).Conclusion: Based on enhanced expression of Ca2+ and attenuated expression of CNX and CRT in patients with chronic ITP, Ca2+ concentration and its associated down-regulated proteins may be important regulatory signals in the pathogenesis of chronic ITP.Keywords: platelets, chronic immune thrombocytopenia, Ca2+, calnexin, calreticulin

Keywords

• platelets
• chronic immune thrombocytopenia
• ca2+
• calnexin
• calreticulin