The Effect of Bacterioruberin Deletion on Production of Bacteriorhodopsin in Halobacterium salinarum R1

Abstract

Bacteriorhodopsin is a retinal protein located in purple membrane of Halobacterium salinarum which acts as light-dependent proton pump. Bacterioruberin is a by-product in bacteriorhodopsin biosynthesis pathway in Halobacterium salinarum. In order to study the effects of bacterioruberin deletion on quantity of active cellular bacteriorhodopsin production, random mutation by UV radiation on Halobacterium salinarum R1 has been carried out. Afterwards, mutated strains which lacked bacterioruberin were selected and production of cellular active bacteriorhodopsin in both mutated and normal (with bacterioruberin) strains were evaluated. The results of this study indicated that the bacterioruberin deletion had insignificant effects on bacteriorhodopsin production. Hence, the biosynthesis pathway of bacteriorhodopsin basically has to be considered independently from the bacterioruberin synthesis.

Keywords

• Bacterioruberin
• Bacteriorhodopsin
• Halobacterium salinarum R1
• UV tolerance
• Absorption spectrum