Chemical Composition of the Essential Oil from Aerial Parts of Javanian Pimpinella pruatjan Molk. and Its Molecular Phylogeny
Agustina D. R. Nurcahyanti,
Issam J. Nasser,
Frank Sporer,
Jürgen Graf,
Nurliani Bermawie,
Jürgen Reichling,
Michael Wink
Affiliations
Agustina D. R. Nurcahyanti
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg D-69120, Germany
Issam J. Nasser
College of Health and Medical Technology, Middle Technical University, Baghdad 10047, Iraq
Frank Sporer
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg D-69120, Germany
Jürgen Graf
Institute of Organic Chemistry, Heidelberg University, Im Neuenheimer Feld 270, Heidelberg D-69120, Germany
Nurliani Bermawie
Department of Plant Genetic Resources and Breeding, Indonesian Spice and Medicinal Crops Research Institute, Indonesian Agency for Agricultural Research and Development (IAARD), Jl. Tentara Pelajar No.3A, Bogor 16111, Indonesia
Jürgen Reichling
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg D-69120, Germany
Michael Wink
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg D-69120, Germany
The species-rich and diverse genus Pimpinella is mainly distributed in Europe and Asia; a few species occur in Africa. Yet, the Javanian Pimpinella, P. pruatjan, which has been used as an aphrodisiac in Indonesian traditional medicine, was studied for the first time in the context of chemical composition, as well as phylogeny analysis and antimicrobial activity. We examined the chemical composition of the essential oil (EO) from aerial parts of P. pruatjan by gas liquid chromatography-mass spectrometry (GLC-MS). The main component of EO was (Z)-γ-bisabolene. Several oxygenated monoterpenes, oxygenated sesquiterpenes, and sesquiterpenes were also detected. The genetic relationship of Pimpinella pruatjan Molk. to other Pimpinella species was reconstructed using nucleotide sequences of the nuclear DNA marker ITS (Internal Transcribed Spacer). P. pruatjan clusters as a sister group to the African Pimpinella species. The EO did not exhibit an apparent antimicrobial activity.
