Concentrations of Acute-Phase Proteins in Milk from Cows with Clinical Mastitis Caused by Different Pathogens
Felipe M. Dalanezi,
Elizabeth M. S. Schmidt,
Sâmea F. Joaquim,
Felipe F. Guimarães,
Simoni T. Guerra,
Bruna C. Lopes,
Ronaldo L. A. Cerri,
Christopher Chadwick,
Hélio Langoni
Affiliations
Felipe M. Dalanezi
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Elizabeth M. S. Schmidt
Department of Veterinary Clinical Science, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Sâmea F. Joaquim
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Felipe F. Guimarães
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Simoni T. Guerra
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Bruna C. Lopes
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Ronaldo L. A. Cerri
Applied Animal Biology, Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
Christopher Chadwick
Life Diagnosis, Inc., West Chester, PA 19380, USA
Hélio Langoni
Department of Animal Production and Preventive Veterinary Medicine, Faculty of Medicine Veterinary and Animal Science, São Paulo State University (UNESP), São Paulo 18168-681, Brazil
Among the new diagnostic methods for mastitis detection under development, milk acute-phase proteins (APPs) are receiving special attention. The study aimed to compare the profile of milk APPs from cows with natural clinical mastitis caused by distinct pathogens. The concentrations of haptoglobin (Hp), serum amyloid A (SAA), alpha-1-acid glycoprotein (AGP), and C-reactive protein (CRP) were measured by Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL). Each APP was compared across the pathogens causing mastitis. The APPs differed statistically (p < 0.05) among the pathogens causing udder infection. There were significant and positive correlations among the concentration profile, for each pathogen, in three of four APPs studied. It can be concluded that the pathogen causing mastitis could modify the profile of release of the APPs in milk. The profile of Hp, AGP, and CRP demonstrated significant correlation, indicating that the three APPs are suggested as biomarkers, in milk, for bovine mastitis.
