Nature Communications (Dec 2022)
Tagging active neurons by soma-targeted Cal-Light
- Jung Ho Hyun,
- Kenichiro Nagahama,
- Ho Namkung,
- Neymi Mignocchi,
- Seung-Eon Roh,
- Patrick Hannan,
- Sarah Krüssel,
- Chuljung Kwak,
- Abigail McElroy,
- Bian Liu,
- Mingguang Cui,
- Seunghwan Lee,
- Dongmin Lee,
- Richard L. Huganir,
- Paul F. Worley,
- Akira Sawa,
- Hyung-Bae Kwon
Affiliations
- Jung Ho Hyun
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Kenichiro Nagahama
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Ho Namkung
- Department of Biomedical Engineering, Johns Hopkins School of Medicine
- Neymi Mignocchi
- Max Planck Florida Institute for Neuroscience
- Seung-Eon Roh
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Patrick Hannan
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Sarah Krüssel
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Chuljung Kwak
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Abigail McElroy
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Bian Liu
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Mingguang Cui
- Department of Anatomy, Korea University College of Medicine
- Seunghwan Lee
- Department of Anatomy, Korea University College of Medicine
- Dongmin Lee
- Department of Anatomy, Korea University College of Medicine
- Richard L. Huganir
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Paul F. Worley
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Akira Sawa
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- Hyung-Bae Kwon
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine
- DOI
- https://doi.org/10.1038/s41467-022-35406-y
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
Techniques for tagging active neurons with high spatiotemporal precision are limited. Here the authors report soma-targeted CalLight (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression, and generate a conditional ST-Cal-Light knock-in mouse.
