Comparative Evaluation of the Foot-and-Mouth Disease Virus Permissive LF-BK α<sub>V</sub>β<sub>6</sub> Cell Line for Senecavirus A Research
Jessica Mason,
Victoria Primavera,
Lauren Martignette,
Benjamin Clark,
Jose Barrera,
Janine Simmons,
William Hurtle,
John G. Neilan,
Michael Puckette
Affiliations
Jessica Mason
SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Victoria Primavera
SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Lauren Martignette
SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Benjamin Clark
U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Jose Barrera
SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Janine Simmons
SAIC, Plum Island Animal Disease Center, Greenport, NY 11944, USA
William Hurtle
U.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA
John G. Neilan
U.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Michael Puckette
U.S. Department of Homeland Security Science & Technology Directorate, Plum Island Animal Disease Center, Greenport, NY 11944, USA
Senecavirus A (SVA) is a member of the family Picornaviridae and enzootic in domestic swine. SVA can induce vesicular lesions that are clinically indistinguishable from Foot-and-mouth disease, a major cause of global trade barriers and agricultural productivity losses worldwide. The LF-BK αVβ6 cell line is a porcine-derived cell line transformed to stably express an αVβ6 bovine integrin and primarily used for enhanced propagation of Foot-and-mouth disease virus (FMDV). Due to the high biosecurity requirements for working with FMDV, SVA has been considered as a surrogate virus to test and evaluate new technologies and countermeasures. Herein we conducted a series of comparative evaluation in vitro studies between SVA and FMDV using the LF-BK αVβ6 cell line. These include utilization of LF-BK αVβ6 cells for field virus isolation, production of high virus titers, and evaluating serological reactivity and virus susceptibility to porcine type I interferons. These four methodologies utilizing LF-BK αVβ6 cells were applicable to research with SVA and results support the current use of SVA as a surrogate for FMDV.
