Controllable blood–brain barrier (BBB) regulation based on gigahertz acoustic streaming

Abstract

The blood–brain barrier (BBB) is a structural and functional barrier necessary for brain homeostasis, and it plays an important role in the realization of neural function and in protecting the brain from damage by circulating toxins and pathogens. However, the extremely dense BBB also severely limits the transport of molecules across it, which is a great hindrance to the diagnosis and treatment of central nervous system (CNS) diseases. This paper reports a new method for controllable opening of the BBB, based on the gigahertz acoustic streaming (AS) generated by a bulk acoustic wave resonant device. By adjusting the input power and working distance of the device, AS with tunable flow rate can be generated to disrupt tight junction proteins (TJs) between endothelial cells. The results obtained with this method show that the gigahertz AS promotes the penetration of dextran molecules with different molecular weights across the BBB. This work provides a new platform for studying the mechanical regulation of BBB by fluid shear forces and a new method for improving the efficiency of drug delivery.