On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation

D. M. Jeziorska; E. A. J. Tunnacliffe; J. M. Brown; H. Ayyub; J. Sloane-Stanley; J. A. Sharpe; B. C. Lagerholm; C. Babbs; A. J. H. Smith; V. J. Buckle; D. R. Higgs

doi:10.1038/s41467-022-33977-4

Nature Communications (Nov 2022)

On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation

D. M. Jeziorska,
E. A. J. Tunnacliffe,
J. M. Brown,
H. Ayyub,
J. Sloane-Stanley,
J. A. Sharpe,
B. C. Lagerholm,
C. Babbs,
A. J. H. Smith,
V. J. Buckle,
D. R. Higgs

Affiliations

D. M. Jeziorska: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
E. A. J. Tunnacliffe: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
J. M. Brown: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
H. Ayyub: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
J. Sloane-Stanley: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
J. A. Sharpe: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
B. C. Lagerholm: Wolfson Imaging Centre, MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
C. Babbs: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
A. J. H. Smith: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
V. J. Buckle: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital
D. R. Higgs: MRC Weatherall Institute for Molecular Medicine, University of Oxford, John Radcliffe Hospital

DOI: https://doi.org/10.1038/s41467-022-33977-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Here the authors observe transcription dynamics in real-time throughout erythropoiesis by combining PP7 tagging of α-globin RNA transcripts and “on-microscope” cell staging. They show how the pattern of transcriptional bursting changes during differentiation, with variability in individual cells significantly reduced at the peak period of gene expression.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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