Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma

Xiaoxv Gao; Chengpeng Sun; Zhenglong Yu; Jian Cang; Xiangge Tian; Xiaokui Huo; Lei Feng; Xinguang Liu; Chao Wang; Baojing Zhang; Xiaochi Ma

Acta Pharmaceutica Sinica B (Mar 2018)

Correlation analysis between the chemical contents and bioactivity for the quality control of Alismatis Rhizoma

Xiaoxv Gao,
Chengpeng Sun,
Zhenglong Yu,
Jian Cang,
Xiangge Tian,
Xiaokui Huo,
Lei Feng,
Xinguang Liu,
Chao Wang,
Baojing Zhang,
Xiaochi Ma

Affiliations

Xiaoxv Gao: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Chengpeng Sun: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Zhenglong Yu: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Jian Cang: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Xiangge Tian: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Xiaokui Huo: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Lei Feng: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Xinguang Liu: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Chao Wang: Corresponding authors. Tel.: +86 411 86110419; fax: +86 411 86110408.; College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Baojing Zhang: College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China
Xiaochi Ma: Corresponding authors. Tel.: +86 411 86110419; fax: +86 411 86110408.; College of Pharmacy, Academy of Integrative Medicine, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China

Journal volume & issue: Vol. 8, no. 2
pp. 242 – 251

Abstract

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In order to clarify regions of production and to discriminate processing methods, quantitative and qualitative analyses for saccharides and terpenes in 35 batches of Alismatis Rhizoma were performed. Methodologies included HPLCPDA, HPLCVWD and UHPLCMSn, combined with principal component analysis (PCA) and partial least squares regression techniques (PLSR). The inhibitory effects of triterpenes and Alismatis Rhizoma extracts on lipase activity were evaluated in vitro. PLSR analysis revealed significant positive correlations (R2 = 0.5795) between the contents of triterpenes 10, 14, 15, 18 and 22 and the inhibitory effects of Alismatis Rhizoma. The present study establishes an effective method for simultaneous determination of multiple components, and identifies key bioactive triterpenes. These results can be used for systematic and novel analytical strategies for the quality control of Alismatis Rhizoma production. KEY WORDS: Alismatis Rhizoma, Qualitative analysis, Saccharide, Terpene, Lipase

Published in Acta Pharmaceutica Sinica B

ISSN: 2211-3835 (Print); 2211-3843 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/acta-pharmaceutica-sinica-b

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