Journal of Functional Biomaterials (Mar 2023)
Magnetic Nanoscalpel for the Effective Treatment of Ascites Tumors
- Tatiana Zamay,
- Sergey Zamay,
- Natalia Luzan,
- Victoriya Fedotovskaya,
- Albert Masyugin,
- Fyodor Zelenov,
- Anastasia Koshmanova,
- Elena Nikolaeva,
- Daria Kirichenko,
- Dmitry Veprintsev,
- Olga Kolovskaya,
- Irina Shchugoreva,
- Galina Zamay,
- Ivan Lapin,
- Anna Lukyanenko,
- Andrey Borus,
- Alexander Sukhachev,
- Mikhail Volochaev,
- Kirill Lukyanenko,
- Alexandr Shabanov,
- Vladimir Zabluda,
- Alexey Zhizhchenko,
- Aleksandr Kuchmizhak,
- Alexey Sokolov,
- Andrey Narodov,
- Vladimir Prokopenko,
- Rinat Galeev,
- Valery Svetlichnyi,
- Anna Kichkailo
Affiliations
- Tatiana Zamay
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Sergey Zamay
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Natalia Luzan
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Victoriya Fedotovskaya
- Laboratory for Biomolecular and Medical Technologies, Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia
- Albert Masyugin
- JSC «NPP «Radiosviaz», Krasnoyarsk 660021, Russia
- Fyodor Zelenov
- JSC «NPP «Radiosviaz», Krasnoyarsk 660021, Russia
- Anastasia Koshmanova
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Elena Nikolaeva
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Daria Kirichenko
- Laboratory for Biomolecular and Medical Technologies, Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia
- Dmitry Veprintsev
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Olga Kolovskaya
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Irina Shchugoreva
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Galina Zamay
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Ivan Lapin
- Laboratory of Advanced Materials and Technology, Siberian Physical Technical Institute, Tomsk State University, Tomsk 634050, Russia
- Anna Lukyanenko
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Andrey Borus
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Alexander Sukhachev
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Mikhail Volochaev
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Kirill Lukyanenko
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Alexandr Shabanov
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Vladimir Zabluda
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Alexey Zhizhchenko
- Institute of Automation and Control Processes (IACP), Far Eastern Branch of the Russian Academy of Science, Vladivostok 690041, Russia
- Aleksandr Kuchmizhak
- Institute of Automation and Control Processes (IACP), Far Eastern Branch of the Russian Academy of Science, Vladivostok 690041, Russia
- Alexey Sokolov
- L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- Andrey Narodov
- Laboratory for Biomolecular and Medical Technologies, Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia
- Vladimir Prokopenko
- V.P. Astafiev Krasnoyarsk State Pedagogical University, Krasnoyarsk 660049, Russia
- Rinat Galeev
- JSC «NPP «Radiosviaz», Krasnoyarsk 660021, Russia
- Valery Svetlichnyi
- Laboratory of Advanced Materials and Technology, Siberian Physical Technical Institute, Tomsk State University, Tomsk 634050, Russia
- Anna Kichkailo
- Federal Research Center “Krasnoyarsk Science Center” of the Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russia
- DOI
- https://doi.org/10.3390/jfb14040179
- Journal volume & issue
-
Vol. 14,
no. 4
p. 179
Abstract
One of the promising novel methods for radical tumor resection at a single-cell level is magneto-mechanical microsurgery (MMM) with magnetic nano- or microdisks modified with cancer-recognizing molecules. A low-frequency alternating magnetic field (AMF) remotely drives and controls the procedure. Here, we present characterization and application of magnetic nanodisks (MNDs) as a surgical instrument (“smart nanoscalpel”) at a single-cell level. MNDs with a quasi-dipole three-layer structure (Au/Ni/Au) and DNA aptamer AS42 (AS42-MNDs) on the surface converted magnetic moment into mechanical and destroyed tumor cells. The effectiveness of MMM was analyzed on Ehrlich ascites carcinoma (EAC) cells in vitro and in vivo using sine and square-shaped AMF with frequencies from 1 to 50 Hz with 0.1 to 1 duty-cycle parameters. MMM with the “Nanoscalpel” in a sine-shaped 20 Hz AMF, a rectangular-shaped 10 Hz AMF, and a 0.5 duty cycle was the most effective. A sine-shaped field caused apoptosis, whereas a rectangular-shaped field caused necrosis. Four sessions of MMM with AS42-MNDs significantly reduced the number of cells in the tumor. In contrast, ascites tumors continued to grow in groups of mice and mice treated with MNDs with nonspecific oligonucleotide NO-MND. Thus, applying a “smart nanoscalpel” is practical for the microsurgery of malignant neoplasms.
Keywords
- magnetic nanodisks
- ascitic tumor
- magneto-mechanical therapy
- “smart nanoscalpel”
- DNA aptamers
- apoptosis