Hydrophilic Scaffolds Containing Extracts of <i>Stryphnodendron adstringens</i> and <i>Abarema cochliacarpa</i> for Wound Healing: In Vivo Proofs of Concept
Maria C. M. A. Alves,
Marismar F. Nascimento,
Bernadeth M. de Almeida,
Matheus M. A. Alves,
Isabel B. Lima-Verde,
Daniela S. Costa,
Daniela C. Medeiros Araújo,
Mariana N. de Paula,
João C. P. de Mello,
Amanda Cano,
Patricia Severino,
Ricardo L. C. de Albuquerque-Júnior,
Eliana B. Souto,
Juliana C. Cardoso
Affiliations
Maria C. M. A. Alves
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Marismar F. Nascimento
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Bernadeth M. de Almeida
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Matheus M. A. Alves
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Isabel B. Lima-Verde
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Daniela S. Costa
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Daniela C. Medeiros Araújo
Department of Pharmacy, Ingá University Centre, Maringá 87035-510, Brazil
Mariana N. de Paula
Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, State University of Maringá, Maringá 87020-900, Brazil
João C. P. de Mello
Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, State University of Maringá, Maringá 87020-900, Brazil
Amanda Cano
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08007 Barcelona, Spain
Patricia Severino
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Ricardo L. C. de Albuquerque-Júnior
Department of Pathology, Federal University of Santa Catarina, Florianópolis 88040-370, Brazil
Eliana B. Souto
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
Juliana C. Cardoso
Post-Graduating Program in Health and Environment, University of Tiradentes, Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
The present work aimed to evaluate the healing effect of hydrophilic polymeric resorbable biomembrane scaffolds containing plant extracts obtained from two different species, both popularly known as Stryphnodendron adstringens or Barbatimão. The hydrogel-based scaffolds were characterized and submitted to biological tests using Wistar rats to evaluate their healing capacity. The wound retraction index and the evaluation of the inflammatory process and tissue collagenization were recorded. The extracts showed antioxidant activity with IC50 between 10 and 20 µg/mL (DPPH assay) and 4–6 mmol Trolox/g (FRAP assay). The extract of Stryphnodendron adstringens (SA) presented gallocatechin, epigallocatechin, and O-methylpigalocatechin, while the extract of Abarema cochliacarpa (AC) presented catechin, dimers of procyanidins, di-O-hydroxide, O-deoxyhexosi-hexoside, and epicatechin. The membranes containing SA extract (GELSA) were more rigid, with a more intense color, but less thick, with a more compact structure and few pores. The membranes containing AC extract (GELAC) presented a mechanical profile like the gelatin membrane (GEL), with greater permeability to water vapor. The GELAC and GELSA membranes showed similar thermal degradation profiles. The wounds treated with the membranes containing the extracts obtained high levels of retraction of the wounds with values around 60% and 80% in three and seven days, respectively. These data indicate that the compounds of both species have promising biological activities in the repair process, showing that the extracts accelerated the healing process due to the lower intensity of the inflammatory reaction and the presence of compounds such as catechin and epigallocatechin.
