Stem Cell Research (Aug 2020)
An integration-free iPSC line ZZUNEUi008-A derived from dermal fibroblasts of a child with cardiac valvular dysplasia carrying a mutation in FLNA gene
- Xiaowei Li,
- Yanyu Lu,
- Jiaxiang Wang,
- Mengduan Liu,
- Mengyu Wang,
- Liang Hu,
- Wenting Du,
- Lu Wang,
- Zhengming Jiang,
- Xiangmei Gu,
- Zhongren Ding,
- Deguang Feng,
- Ling Li,
- Jianzeng Dong,
- Xiaoyan Zhao
Affiliations
- Xiaowei Li
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Yanyu Lu
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Jiaxiang Wang
- Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Mengduan Liu
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Mengyu Wang
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Liang Hu
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Wenting Du
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Lu Wang
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Zhengming Jiang
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Xiangmei Gu
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Zhongren Ding
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China
- Deguang Feng
- Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Ling Li
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Jianzeng Dong
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China; Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China; Corresponding authors at: The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Longhu Zhonghuan Road No. 1, Jinshui District, Zhengzhou 450052, China.
- Xiaoyan Zhao
- Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China; Corresponding authors at: The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Longhu Zhonghuan Road No. 1, Jinshui District, Zhengzhou 450052, China.
- Journal volume & issue
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Vol. 47
p. 101882
Abstract
FLNA gene encodes an actin-binding protein filamin A and mutations in FLNA can causes X-Linked cardiac valvular dysplasia. In this study, we report the generation of ZZUNEUi008-A, a human induced pluripotent stem cell line from a 10-year-old male patient with c. 84G → A in FLNA gene using non-integrative Sendai viral reprogramming technology. The ZZUNEUi008-A iPSC line expresses pluripotency markers, exhibits a normal male karyotype (46, XY) and can differentiate into three germ layers in vivo.
