The Associations between Leaf Morphology, Phenylalanine Ammonia Lyase Activity, Reactive Oxygen Species, and Fusarium Resistance in Selected Species of Wheat with Different Ploidy Levels

Abstract

In wheat, resistance to Fusarium is conditioned by anatomical, morphological, and physiological traits. The aim of this study was to evaluate selected elements of constitutive barriers in common wheat, spelt, Polish wheat, emmer, and einkorn. The activity of the phenylalanine ammonia-lyase (PAL) enzyme and rate of reactive oxygen species (ROS) production were evaluated in the tissues of common wheat and spelt inoculated with Fusarium culmorum. Most of the relict wheat species were more abundant in morphological barriers than common wheat. F. culmorum penetrated constitutive barriers, which increased PAL activity and intensified ROS production 24 h after inoculation in wheat tissues. The lowest increase in PAL activity after inoculation was observed in cv. Sumai3, which resistance is based on limiting the spread of F. culmorum within the spike. Spelt line Tas 581 glumes were characterized by the highest concentration of ROS 24 h after inoculation. The ROS content remained high for five days. The results of this study indicate that high trichome density plays a key role in resistance to pathogens. In the resistant spelt line with effective constitutive barriers, PAL activity and ROS content were higher than those observed in susceptible wheats after inoculation with F. culmorum.

Keywords

• wheat
• phenylalanine ammonia-lyase
• reactive oxygen species
• <i>Fusarium culmorum</i>