Cailiao Baohu (Jan 2023)

Growth Characteristics of Sulfate Reducing Bacteria in Oxygenated Pipeline of Shale Gas Field

  • QIN Min, LIAO Ke-xi, BAI Yang, LIAO Cheng-long, WEI Meng-jiao

DOI
https://doi.org/10.16577/j.issn.1001-1560.2023.0005
Journal volume & issue
Vol. 56, no. 1
pp. 30 – 34

Abstract

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For clarifying the growth characteristics of sulfate reducing bacteria in shale gas pipelines, water samples were collected from a shale gas-gathering pipeline, and the sulfate reducing bacteria(SRB) in the samples were enriched and purified. The DNA of SRB was extracted, and then the gene amplification technology(Polymerase Chain Reaction, PCR) was utilized to describe the characteristics of SRB community. Furthermore, the growth characteristics of SRB in dissolved oxygen environment were quantitatively tested. Results showed that the SRB in shale gas gathering pipeline were mainly Bacteroidota, Firmicutes and Desulfobacterota. The concentration of dissolved oxygen had an impact on the growth and metabolism of SRB. Under a dissolved oxygen concentration of 0~2.5 mg/L, SRB could still grow and reproduce in large quantities. Furthermore, under a dissolved oxygen concentration of 4.5 mg/L and above, the growth and metabolism of SRB were completely inhibited, indicating that SRB were able to grow and reproduce under low O2 concentration in the shale gas gathering pipeline.

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