Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion

Zhao Zhao; Jinglin Fu; Soma Dhakal; Alexander Johnson-Buck; Minghui Liu; Ting Zhang; Neal W. Woodbury; Yan Liu; Nils G. Walter; Hao Yan

doi:10.1038/ncomms10619

Nature Communications (Feb 2016)

Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion

Zhao Zhao,
Jinglin Fu,
Soma Dhakal,
Alexander Johnson-Buck,
Minghui Liu,
Ting Zhang,
Neal W. Woodbury,
Yan Liu,
Nils G. Walter,
Hao Yan

Affiliations

Zhao Zhao: Center for Molecular Design and Biomimetics, the Biodesign Institute at Arizona State University
Jinglin Fu: Department of Chemistry, Center for Computational and Integrative Biology, Rutgers University-Camden
Soma Dhakal: Department of Chemistry, Single Molecule Analysis Group, University of Michigan
Alexander Johnson-Buck: Department of Chemistry, Single Molecule Analysis Group, University of Michigan
Minghui Liu: Center for Molecular Design and Biomimetics, the Biodesign Institute at Arizona State University
Ting Zhang: Department of Chemistry, Center for Computational and Integrative Biology, Rutgers University-Camden
Neal W. Woodbury: School of Molecular Sciences, Arizona State University
Yan Liu: Center for Molecular Design and Biomimetics, the Biodesign Institute at Arizona State University
Nils G. Walter: Department of Chemistry, Single Molecule Analysis Group, University of Michigan
Hao Yan: Center for Molecular Design and Biomimetics, the Biodesign Institute at Arizona State University

DOI: https://doi.org/10.1038/ncomms10619
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 9

Abstract

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Cells compartmentalize enzymes for enhanced efficiency of their metabolic pathways. Here, the authors describe a self-assembly approach to construct DNA nanocaged enzymes for enhancing catalytic activity and stability, and observe an inversed correlation between the protein size and the activity enhancement.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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