Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis
Ramona Kuhn,
Jörg Böllmann,
Kathrin Krahl,
Isaac Mbir Bryant,
Marion Martienssen
Affiliations
Ramona Kuhn
Corresponding author.; Brandenburg University of Technology Cottbus-Senftenberg, Institute of Environmental Technology, Chair of Biotechnology of Water Treatment, 03046 Cottbus, Germany
Jörg Böllmann
Brandenburg University of Technology Cottbus-Senftenberg, Institute of Environmental Technology, Chair of Biotechnology of Water Treatment, 03046 Cottbus, Germany
Kathrin Krahl
Brandenburg University of Technology Cottbus-Senftenberg, Institute of Environmental Technology, Chair of Biotechnology of Water Treatment, 03046 Cottbus, Germany
Isaac Mbir Bryant
Brandenburg University of Technology Cottbus-Senftenberg, Institute of Environmental Technology, Chair of Biotechnology of Water Treatment, 03046 Cottbus, Germany
Marion Martienssen
Brandenburg University of Technology Cottbus-Senftenberg, Institute of Environmental Technology, Chair of Biotechnology of Water Treatment, 03046 Cottbus, Germany
The data presented in this article provide supporting information to the related research article “Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples” (Kuhn et al., 2017) [1]. In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water. Keywords: Cost analysis, DNA sample load, Gel electrophoresis