Multimodal interference-based imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm

Scott Gladstein; Luay M. Almassalha; Lusik Cherkezyan; John E. Chandler; Adam Eshein; Aya Eid; Di Zhang; Wenli Wu; Greta M. Bauer; Andrew D. Stephens; Simona Morochnik; Hariharan Subramanian; John F. Marko; Guillermo A. Ameer; Igal Szleifer; Vadim Backman

doi:10.1038/s41467-019-09717-6

Nature Communications (Apr 2019)

Multimodal interference-based imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm

Scott Gladstein,
Luay M. Almassalha,
Lusik Cherkezyan,
John E. Chandler,
Adam Eshein,
Aya Eid,
Di Zhang,
Wenli Wu,
Greta M. Bauer,
Andrew D. Stephens,
Simona Morochnik,
Hariharan Subramanian,
John F. Marko,
Guillermo A. Ameer,
Igal Szleifer,
Vadim Backman

Affiliations

Scott Gladstein: Department of Biomedical Engineering, Northwestern University
Luay M. Almassalha: Department of Biomedical Engineering, Northwestern University
Lusik Cherkezyan: Department of Biomedical Engineering, Northwestern University
John E. Chandler: Department of Biomedical Engineering, Northwestern University
Adam Eshein: Department of Biomedical Engineering, Northwestern University
Aya Eid: Department of Biomedical Engineering, Northwestern University
Di Zhang: Department of Biomedical Engineering, Northwestern University
Wenli Wu: Department of Biomedical Engineering, Northwestern University
Greta M. Bauer: Department of Biomedical Engineering, Northwestern University
Andrew D. Stephens: Department of Molecular Biosciences, Northwestern University
Simona Morochnik: Department of Biomedical Engineering, Northwestern University
Hariharan Subramanian: Department of Biomedical Engineering, Northwestern University
John F. Marko: Department of Molecular Biosciences, Northwestern University
Guillermo A. Ameer: Department of Biomedical Engineering, Northwestern University
Igal Szleifer: Department of Biomedical Engineering, Northwestern University
Vadim Backman: Department of Biomedical Engineering, Northwestern University

DOI: https://doi.org/10.1038/s41467-019-09717-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 15

Abstract

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Methods to track molecular motion in eukaryotic cells mostly rely on fluorescent labels, transfection or photobleaching. Here the authors use multimodal partial wave spectroscopy to perform label-free live cell measurements of nanoscale structure and macromolecular motion with millisecond temporal resolution.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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