CopA3 Peptide Prevents Ultraviolet-Induced Inhibition of Type-I Procollagen and Induction of Matrix Metalloproteinase-1 in Human Skin Fibroblasts
Dong-Hee Kim,
Han-Hyuk Kim,
Hyeon-Jeong Kim,
Hyun-Gug Jung,
Jae-Myo Yu,
Eun-Su Lee,
Yong-Hun Cho,
Dong-In Kim,
Bong-Jeun An
Affiliations
Dong-Hee Kim
Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Korea
Han-Hyuk Kim
Advanced Medical Fusion Textile Center, Gyeongbuk Technopark Foundation, Gyeongsan 712-210, Korea
Hyeon-Jeong Kim
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Hyun-Gug Jung
Radiation Division for Biotechnology, Advanced Radiation Technology Institute, Jeongeup Campus of Atomic Energy Research Institute (KAERI), Jeonbuk 580-185, Korea
Jae-Myo Yu
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Eun-Su Lee
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Yong-Hun Cho
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Dong-In Kim
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Bong-Jeun An
Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 712-715, Korea
Ultraviolet (UV) exposure is well-known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. A 9-mer peptide, CopA3 (CopA3) was synthesized from a natural peptide, coprisin, which is isolated from the dung beetle Copris tripartitus. As part of our continuing search for novel bioactive natural products, CopA3 was investigated for its in vitro anti-skin photoaging activity. UV-induced inhibition of type-I procollagen and induction of MMP-1 were partially prevented in human skin fibroblasts by CopA3 peptide in a dose-dependent manner. At a concentration of 25 μM, CopA3 nearly completely inhibited MMP-1 expression. These results suggest that CopA3, an insect peptide, is a potential candidate for the prevention and treatment of skin aging.
