Optimalisation de l'extraction d'ADN génomique de la morelle jaune (Solanum elaeagnifolium Cav.), une plante invasive des milieux cultivés en région méditerranéenne

Abstract

Optimization of the genomic DNA extraction method of silverleaf nightshade (Solanum elaeagnifolium Cav.), an invasive plant in the cultivated areas within the Mediterranean region. The geographical origin of an invasive plant in the cultivated area within the Mediterranean region, silverleaf nightshade, Solanum elaeagnifolium Cav. (Solanaceae) should be identified through the analysis of genetic similarities between native and introduced populations using microsatellite markers. Beforehand, an effective and less costly method for extracting genomic DNA from dehydrated and polysaccharide-rich leaves was investigated. The original Doyle's protocol based on the use of a cationic detergent, CTAB, was successfully optimized in order to extract high quality DNA (A260/280 ratio of 1.8; A260/230 ratio of 1.7 to 2). The DNA yields obtained with this new protocol were higher than those obtained with a commercial kit, although quality of the DNA extracted was comparable. The improvement of the protocol was further proven by the amplification of several microsatellite loci first developed in eggplant, Solanum melongena L.

Keywords

• DNA
• invasive species
• Solanum elaeagnifolium
• microsatellites
• leaf tissue
• Nanodrop