Wound tissue remodeling by latex exudate of Himatanthus drasticus: A plant species used in Brazilian folk medicine
Tamiris F.G. Souza,
Márcio V. Ramos,
Taiana M. Pierdoná,
Liviane M.A. Rabelo,
Mirele S. Vasconcelos,
Luana D. Carmo,
Gisele F.P. Rangel,
Yuri T.C.N. Paiva,
Emilia T. Sousa,
Ingrid S.T. Figueiredo,
Nylane M.N. Alencar
Affiliations
Tamiris F.G. Souza
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Márcio V. Ramos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil; Corresponding author.
Taiana M. Pierdoná
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil; Faculty of Kinesiology and Recreation Management, Children's Hospital Research Institute of Manitoba (CHRIM), University of Manitoba, Winnipeg, Manitoba, Canada
Liviane M.A. Rabelo
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Mirele S. Vasconcelos
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil; Instituto Federal de Educação Ciência e Tecnologia do Ceara (IFCE), Campus Baturité, Ceará, Brazil
Luana D. Carmo
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Gisele F.P. Rangel
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Yuri T.C.N. Paiva
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Emilia T. Sousa
Departamento de Patologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil
Ingrid S.T. Figueiredo
Centro Universitário Estácio de Sá, Fortaleza, CE, Brazil
Nylane M.N. Alencar
Núcleo de Pesquisa e Desenvolvimento de Medicamentos (NPDM), Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil; Corresponding author. Tel.: +55-85-33668339; Fax: +55-85-988984835.
This work investigated the healing properties of proteins extracted of latex (HdLP) on excisional wounds. Cell toxicity of HdLP was investigated carried out in murine fibroblasts after incubation with HdLP (12.5–100 μg/ml). The dermal irritability test was performed to evaluate dermal reactions. The wounds were performed and treated with vehicle or HdLP (0.5 %, 1.0 %, and 2.0 %). The macroscopic parameters, histological analysis and measurement of inflammatory markers and mediators were evaluated. HdLP did not exhibit cytotoxicity and did not induce skin irritation. HdLP stimulated the release of IL-1β at the beginning of the inflammatory phase. This effect probably favored the earlier release of IL-10 by macrophages, during the proliferative phase. The shortening and completeness of healing were characterized by fibroblast proliferation and the presence of newly synthesized collagen fibers. This was accompanied by well-organized re-epithelialization. The involvement of latex proteins in this activity is reported for the first time.
