The Evaluation of Vitamin E and TiO2 Nanoparticles Administration in Parkinson’s Rat Model

Abstract

Objective: Parkinson’s disease (PD) is a severely debilitating disease for which no suitable treatment has been foundso far. In recent years, nanoparticles (NPs) have shown therapeutic potential in PD. Thus, in the current research, forthe first time, we investigated the effects of vitamin E and TiO2 nanoparticles (TiO2-NPs) on a rat model of PD.Materials and Methods: In this experimental study, after preparation and induction of PD, rats were administratedwith vitamin E and TiO2-NPs. One day after receiving the last dose of treatments, rats were killed and substantianigra was extracted from the brain and its cell suspension was prepared. In each group, female rats were mated, andafter confirmation that the female rats were pregnant by vaginal smear test, the fetus was removed. Cell viability wasstudied by the MTT method and apoptosis, and necrosis were studied by the flow cytometry technique. Also, after RNAextraction and cDNA synthesis, the expression of Bcl-2 and circRNA 0001518 genes were studied using the real timepolymerase chain reaction (RT-PCR) technique. For analyzing the data, two-way ANOVA was used.Results: The results showed a sharp decrease in cell viability in female PD rats and fetuses resulting from PD femalerats. Vitamin E treatment showed the greatest effect on increasing cell viability. Decreased expression of the Bcl-2 geneand increased expression of circRNA 0001518 were observed in PD conditions.Conclusion: Administration of vitamin E may be a good option for reducing PD-induced cell death.

Keywords

• parkinson' s disease
• vitamin e
• cyclic rnas
• nanoparticle
• tio2