Emerging Intestinal Microsporidia Infection in HIV(+)/AIDS Patients in Iran: Microscopic and Molecular Detection

Abstract

Background: Species of Microsporidia have been known as opportunistic obli-gate intracellular parasites particularly in immunocompromised patients. Enterocyto-zoon bieneusi is one of most prevalent intestinal microsporida parasites in HIV+/AIDS patients. In this study, intestinal microsporidia infection was deter-mined in HIV+/AIDS patients using microscopic and molecular methods. Methods: Stool samples were collected from HIV+/AIDS patients during 12 months. All of the stool specimens washed with PBS (pH: 7.5). Slim slides were prepared from each sample and were examined using light microscope with 1000X magnification. DNA extraction carried out in microscopic positive samples. DNA amplification and genus/species identification also performed by Nested-PCR and sequencing techniques. Results: From 81 stool samples, 25 were infected with microsporidia species and E. bieneusi were identified in all of positive samples. No Encephalitozoon spp. was identified in 81 collected samples using specific primers. Conclusion: E. bieneusi is the most prevalent intestinal microsporidia in immuno-compromised patients of Iran. On the other hand, Nested-PCR using specific pri-mers for ssu rRNA gene is an appropriate molecular method for identification of E. bieneusi.

Keywords

• Encephalitozoon spp
• Enterocytozoon bieneusi
• HIV /AIDS patients
• Iran
• Nested-PCR