A Simple HPTLC Method for Simultaneous Estimation of Atenolol and Chlorthalidone in Pharmaceuticals

Abstract

A new simple, precise, and accurate, high-performance thin-layer chromatography method was developed for the simultaneous determination of Atenolol and Chlorthalidone in tablet dosage forms. In this method, TLC aluminum plates pre-coated with silica gel 60 F254used as the stationary phase. The mobile phase consisting of Methylene Chloride: methanol: Ammonia solution 25%, in the ratio of 8.8:1.3:0.1 v/v is used. The densitometry evaluation was done at wavelength 266 nm using Camag scanner III. The developed method shows a well resolution between two peaks Atenolol and Chlorthalidone with Rf values 0.115 (±0.03) and 0.458(±0.03), respectively. The proposed method was validated as per International Conference on Harmonization (ICH) guidelines for parameters such as specificity, precision, accuracy, linearity, and ruggedness and reported the values. The linear regression analysis shows that the correlation coefficient R2 value for Atenolol is 0.999 and 0.997, respectively. The mean percentage recovery values obtained for Atenolol is 101.4 and for Chlorthalidone is 98.4. The statistical analysis shows that this method is suitable for routine analysis for quantification of Atenolol and Chlorthalidone in single or combined dosage forms of formulations.

Keywords

• Chlorthalidone
• Atenolol
• Validation
• densitometry
• High-performance thin-layer chromatography (HPTLC)