Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics

Abstract

Summary: The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings.For complete details on the use and execution of this protocol, please refer to Page et al. (2022).

Keywords

• Immunology
• Microbiology
• Molecular Biology