Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

Sheng-Yu Huang; Chih-Heng Huang; Chi-Jene Chen; Ting-Wen Chen; Chun-Yuan Lin; Yueh-Te Lin; Shu-Ming Kuo; Chung-Guei Huang; Li-Ang Lee; Yi-Hsiang Chen; Mei-Feng Chen; Rei-Lin Kuo; Shin-Ru Shih

Molecular Therapy: Nucleic Acids (Sep 2019)

Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus

Sheng-Yu Huang,
Chih-Heng Huang,
Chi-Jene Chen,
Ting-Wen Chen,
Chun-Yuan Lin,
Yueh-Te Lin,
Shu-Ming Kuo,
Chung-Guei Huang,
Li-Ang Lee,
Yi-Hsiang Chen,
Mei-Feng Chen,
Rei-Lin Kuo,
Shin-Ru Shih

Affiliations

Sheng-Yu Huang: Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Chih-Heng Huang: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; The Institute of Microbiology and Immunology, National Defense Medical Center, Taipei 11490, Taiwan; The Institute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan
Chi-Jene Chen: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Ting-Wen Chen: Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu 30068, Taiwan; Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30068, Taiwan
Chun-Yuan Lin: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Taoyuan 33302, Taiwan
Yueh-Te Lin: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Taoyuan 33302, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Shu-Ming Kuo: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Chung-Guei Huang: Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan
Li-Ang Lee: Department of Otorhinolaryngology-Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; Faculty of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Yi-Hsiang Chen: Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Mei-Feng Chen: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Rei-Lin Kuo: Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan 33302, Taiwan
Shin-Ru Shih: Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan; Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan; Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan; Corresponding author: Shin-Ru Shih, PhD, Research Center for Emerging Viral Infections, College of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.

Journal volume & issue: Vol. 17
pp. 10 – 23

Abstract

Read online

The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3′ UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication. Keywords: influenza A virus, miR-1290, vimentin, viral ribonucleoprotein, vRNP, miRNA, virus, host species-specificity, ferret

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

About the journal