高油酸花生油酶促酯交换制备1,3- 二棕榈酸-2-油酸甘油三酯Preparation of 1,3-dipalmitoyl-2-oleoylglycerol with high-oleic peanut oil by enzymatic transesterification

Abstract

旨在为可可脂生产提供新的基料油，同时为高油酸花生油的高值化利用提供研究基础，以高油酸花生油和棕榈酸乙酯为原料，脂肪酶NS40086为催化剂，在无溶剂体系下酶法合成1,3-二棕榈酸-2-油酸甘油三酯（POP）。采用单因素实验研究反应条件对POP含量、酰基位移、sn-2位油酸相对含量的影响，并采用响应面法进行优化。结果表明：高油酸花生油酶促酯交换制备POP的最佳反应条件为反应时间3 h、底物（棕榈酸乙酯与高油酸花生油）物质的量比11∶ 1、酶添加量（以底物总质量计）3%、反应温度50 ℃，在此条件下POP含量为86.48%，酰基位移为3.25%，sn-2位油酸相对含量为72.88%。In order to provide new base oil for the production of cocoa butter and research basis for the high-value utilization of high-oleic peanut oil, with high-oleic peanut oil and ethyl palmitate as raw materials, NS40086 lipase as catalyst, 1,3-dipalmitoyl-2-oleoylglycerol (POP) was synthesized by enzymatic transesterification in a solvent-free system. The effects of reaction conditions on the POP content, acyl migration and the relative content of sn-2 oleic acid were studied by single factor experiment, and response surface methodology was used to determine the optimal reaction conditions. The results showed that the optimal reaction conditions were obtained as follows: reaction time 3 h, molar ratio of ethyl palmitate to high-oleic peanut oil 11∶ 1, dosage of enzyme 3% (based on total substrates mass), reaction temperature 50 ℃. Under these conditions, the POP content, acyl migration and the relative content of sn-2 oleic acid were 86.48%, 3.25% and 72.88%, respectively.

Keywords

• 酶促酯交换；高油酸花生油；1，3-二棕榈酸-2-油酸甘油三酯；酰基位移；响应面法