The gene polymorphisms of eNOS and MTHFR modulates the development of preeclampsia in Han population
Jinghong Rao,
Youfang Chen,
Xuan Chen,
Ruoying Wu,
Shimu Luo,
Qingfan Lin,
Zhenzhu Dong,
Jingying Huang
Affiliations
Jinghong Rao
Department of Obstetrics and Gynecology, Quanzhou First Hospital Affiliated to Fujian Medical University, No.250 East Street, Quanzhou 362000, Fujian, China
Youfang Chen
Department of Clinical Medicine, Quanzhou Medical College, Quanzhou 362011, Fujian, China
Xuan Chen
Department of Obstetrics and Gynecology, Quanzhou First Hospital Affiliated to Fujian Medical University, No.250 East Street, Quanzhou 362000, Fujian, China; Corresponding author.
Ruoying Wu
Double Medical Technology Inc. No. 18, Shanbianhong East Road, Haicang District, Xiamen 361026, Fujian, China
Shimu Luo
Clinical Laboratory, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou 362011, Fujian, China
Qingfan Lin
Department of Clinical Medicine, Quanzhou Medical College, Quanzhou 362011, Fujian, China
Zhenzhu Dong
Department of Obstetrics and Gynecology, Quanzhou First Hospital Affiliated to Fujian Medical University, No.250 East Street, Quanzhou 362000, Fujian, China
Jingying Huang
Department of Obstetrics and Gynecology, Quanzhou First Hospital Affiliated to Fujian Medical University, No.250 East Street, Quanzhou 362000, Fujian, China
We explored the effects of the endothelial nitric oxide synthase (eNOS) gene −786 T > C and 894 G > T locus polymorphisms and the methylenetetrahydrofolate reductase (MTHFR) gene 1298 A > C and 677 C > T locus polymorphisms on preeclampsia (PE) in pregnant women in Quanzhou area and provide reliable and stable predictors PE. This study included 160 normal pregnant women (normal control group) and 160 women with preeclampsia (PE group). Polymorphisms in eNOS gene and MTHFR were analyzed by the polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) technique. eNOS 894 G > T locus and MTHFR 1298 A > C locus had no significant difference between the two groups. In the PE patients, eNOS −786 T allele (OR: 2.07, p = 0.03) and MTHFR 677 C allele (OR: 1.83, p = 0.04) had significantly lower frequency. The nitric oxide (NO) level in patients with eNOS −786 C C was significantly lower than that in those with −786 TT. The homocysteine (Hcy) level in patients with MTHFR 677 TT was significantly higher than that in those with 677 C C. In conclusion, the frequency of the eNOS −786 C C genotype and MTHFR 677 TT genotype are higher in women with PE, which cause lower NO level and higher Hcy level.
