RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

Abstract

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.