RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells

Yoshio Bando; Taiichi Katayama; Manabu Taniguchi; Tomohiko Ishibashi; Noriyuki Matsuo; Satoshi Ogawa; Masaya Tohyama

Neurobiology of Disease (Feb 2005)

RA410/Sly1 suppresses MPP+ and 6-hydroxydopamine-induced cell death in SH-SY5Y cells

Yoshio Bando,
Taiichi Katayama,
Manabu Taniguchi,
Tomohiko Ishibashi,
Noriyuki Matsuo,
Satoshi Ogawa,
Masaya Tohyama

Affiliations

Yoshio Bando: Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan; Corresponding author. Department of Anatomy, Asahikawa Medical College, Midorigaoka-Higashi 2-1-1-1, Asahikawa, Hokkaido 078-8510, Japan. Fax: +81 166 68 2309.
Taiichi Katayama: Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology (CREST, JST), Kawaguchi, Japan
Manabu Taniguchi: Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan
Tomohiko Ishibashi: Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan
Noriyuki Matsuo: Licensing Division, Dainippon Pharmaceutical Co. Ltd., Osaka, Japan
Satoshi Ogawa: Core Research for Evolutional Science and Technology, Japan Science and Technology (CREST, JST), Kawaguchi, Japan; Department of Neuroanatomy, School of Medicine, Kanazawa University, Kanazawa, Japan
Masaya Tohyama: Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology (CREST, JST), Kawaguchi, Japan

Journal volume & issue: Vol. 18, no. 1
pp. 143 – 151

Abstract

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Parkinson's disease is characterized by selective loss of dopaminergic neurons in the substantia nigra. However, its associated cell death mechanism remains unknown. 1-Methyl-4-phenil-pyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) cause dopaminergic neuronal cell death. Both are widely used to model PD. We investigated the role of a vesicle-transport-related protein, RA410/Sly1, in SH-SY5Y cells to clarify the mechanism of cellular adaptation to MPP+ and 6-OHDA-induced stress. Antisense RA410/Sly1 transformants treated with these toxins displayed reduced viability in comparison with viability of wild-type or RA410/Sly1 sense transformants. Electron microscopy analysis indicated that the ER in MPP+-treated antisense RA410/Sly1 transformants was rapidly disrupted in comparison to wild-type or sense RNA transformants. Cell death induced by MPP+ and 6-OHDA was suppressed in RA410/Sly1 sense transformants through suppression of caspase-2, -3 and -9 activation. These results suggest that RA410/Sly1 plays an important cytoprotective role in MPP+ and 6-OHDA-induced cellular perturbation.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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