Communications Biology (Mar 2024)

Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

  • Chao Ren,
  • Senyue Hao,
  • Fei Wang,
  • Abigail Matt,
  • Marcello Magri Amaral,
  • Daniel Yang,
  • Leyao Wang,
  • Chao Zhou

DOI
https://doi.org/10.1038/s42003-024-05973-5
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 8

Abstract

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Abstract Dynamic contrast optical coherence tomography (DyC-OCT), an emerging imaging method, utilizes fluctuation patterns in OCT signals to enhance contrast, thereby enabling non-invasive label-free volumetric live cell imaging. In this mini review, we explain the core concepts behind DyC-OCT image formation and its system configurations, serving as practical guidance for future DyC-OCT users. Subsequently, we explore its applications in delivering high-quality, contrast-enhanced images of cellular morphology, as well as in monitoring changes in cellular activity/viability assay experiments.