Scientific Reports (Nov 2022)

Introgression of tsv1 improves tungro disease resistance of a rice variety BRRI dhan71

  • Tapas Kumer Hore,
  • Mary Ann Inabangan-asilo,
  • Ratna Wulandari,
  • Mohammad Abdul Latif,
  • Sheikh Arafat Islam Nihad,
  • Jose E. Hernandez,
  • Glenn B. Gregorio,
  • Teresita U. Dalisay,
  • Maria Genaleen Q. Diaz,
  • Balachiranjeevi Ch.,
  • B. P. Mallikarjuna Swamy

DOI
https://doi.org/10.1038/s41598-022-23413-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Abstract Rice Tungro disease poses a threat to rice production in Asia. Marker assisted backcross breeding is the most feasible approach to address the tungro disease. We targeted to introgress tungro resistance locus tsv1 from Matatag 1 into a popular but tungro susceptible rice variety of Bangladesh, BRRI dhan71. The tsv1 locus was traced using two tightly linked markers RM336 and RM21801, and background genotyping was carried out using 7 K SNPs. A series of three back crosses followed by selfing resulted in identification of plants similar to BRRI dhan71. The background recovery varied at 91–95% with most of the lines having 95%. The disease screening of the lines showed moderate to high level of tungro resistance with a disease index score of ≤ 5. Introgression Lines (ILs) had medium slender grain type, and head rice recovery (59.2%), amylose content (20.1%), gel consistency (40.1 mm) and gelatinization temperature were within the acceptable range. AMMI and Kang’s stability analysis based on multi-location data revealed that multiple selected ILs outperformed BRRI dhan71 across the locations. IR144480-2-2-5, IR144483-1-2-4, IR144484-1-2-2 and IR144484-1-2-5 are the most promising lines. These lines will be further evaluated and nominated for varietal testing in Bangladesh.