Study on extraction process of methylated catechin from Zijuan tea by response surface methodology

Abstract

Objective: optimizing the extraction process of methylated catechin from Zijuan tea. Methods: Using acid ethanol as extraction solvent, based on three single factor experiments, the extraction time, extraction temperature and liquid-solid ratio were used to investigate the interaction of the above three factors on the extraction rate of methylated catechin from Zijuan tea. Results: The optimum extraction conditions were as follows: extraction time 100 min, extraction temperature 75 ℃, solid-liquid ratio（m Zijuan tea∶VSolvent） 1∶74 （g/mL）; under these conditions, the mass concentration of methylated catechin in the extract could reach 26.7 mg/g, and the relative error with the verification value was 1.48%. Conclusion: HPLC and response surface methods were used to optimize the extraction process of methylated catechins in Zijuan tea. The process is simple and the verification results are reasonable.

Keywords

• zijuan tea
• response surface methodology
• high performance liquid chromatography
• methylated catechin
• extraction rate