Evaluating the antimicrobial activity and antitumor screening of green synthesized silver nanoparticles compounds, using Syzygium jambolanum, towards MCF7 cell line (Breast cancer cell line)

Abstract

In this study, rapid, simple approach was applied for synthesis of silver nanoparticles using S. jambolanum aqueous leaf extract. The plant extract acts both as a reducing agent as well as capping agent. To identify the compounds responsible for reduction of silver ions, the functional groups present in plant extract were investigated by FTIR. Various techniques used to characterize synthesized nanoparticles are UV Visible spectrophotometer, Scanning Electron Microscopy (SEM), Energy dispersive X-ray analyser (EDX). UV-Visible spectrophotometer showed absorbance peak in the range of 335 nm. The NPs displayed higher antioxidant activity, antibacterial activities against both gram positive and gram negative bacteria. These NPs also exhibited effective anti-cancerous activity with a median inhibitory concentration (IC50) of 0.2588 ppm/100µL against MCF-7 breast cancer cell line in cytotoxic assay and indicated antiheamolytic activity at less concentration. Results confirmed this protocol as simple, raspid, one step, eco-friendly, nontoxic and an alternative conventional physical/chemical method. Only 15 min were required for the conversion of silver ions into silver nanoparticles at room temperature, without the involvement of any hazardous chemical.

Keywords

• Syzygium jambolanum
• Silver nanoparticles characterization (UV-Vis, SEM, FTIR, Zeta potential)
• Antimicrobial activity
• Haemolytic activity
• Antioxidant activity
• Anticancer activity