Frontiers in Microbiology (Feb 2024)

Porcine alveolar macrophages host proteins interacting with African swine fever virus p72

  • Zhijun Weng,
  • Zhijun Weng,
  • Xiaoyu Zheng,
  • Xiaoyu Zheng,
  • Yifan Liang,
  • Yifan Liang,
  • Xiongnan Chen,
  • Xiongnan Chen,
  • Qin Peng,
  • Qin Peng,
  • Guihong Zhang,
  • Guihong Zhang,
  • Guihong Zhang,
  • Lang Gong,
  • Lang Gong,
  • Lang Gong,
  • Zezhong Zheng,
  • Zezhong Zheng,
  • Zezhong Zheng

DOI
https://doi.org/10.3389/fmicb.2024.1370417
Journal volume & issue
Vol. 15

Abstract

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IntroductionAfrican swine fever virus (ASFV) is a highly contagious virus that spreads rapidly and has a mortality rate of up to 100% in domestic pigs, leading to significant economic losses in the pig industry. The major capsid protein p72 of ASFV plays a critical role in viral invasion and immune evasion.MethodsIn this study, we used yeast two-hybrid screening to identify host proteins interacting with p72 in porcine alveolar macrophages (PAMs) and verified these proteins using confocal microscopy and immunoprecipitation techniques.Results and DiscussionWe validated 13 proteins that interact with p72, including CD63, B2M, YTHDF2, FTH1, SHFL, CDK5RAP3, VIM, PELO, TIMP2, PHYH, C1QC, CMAS, and ERCC1. Enrichment analysis and protein-protein interaction network analysis of these interacting proteins revealed their involvement in virus attachment, invasion, replication, assembly, and immune regulation. These findings provide new insights into the function of p72 and valuable information for future research on the interaction between ASFV and host proteins.

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