Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.
Juan Marzoa,
Sandra Sánchez,
Charlotte Brookes,
Stephen Taylor,
Andrew Gorringe,
M. Teresa Criado,
Carlos M. Ferreirós
Affiliations
Juan Marzoa
University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, Spain
Sandra Sánchez
University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, Spain
Charlotte Brookes
Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.
Stephen Taylor
Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.
Andrew Gorringe
Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.
M. Teresa Criado
University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, Spain
Carlos M. Ferreirós
University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, Spain
Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap) detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP), which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity.
