Control of lateral root initiation by DA3 in Arabidopsis
Yuancheng Peng,
Shan Jiang,
Jiayu Wang,
Xuewei Xu,
Ximing Gong,
Weihuan Jin,
Chunxia Song,
Zhiqiang Dong,
Siyu Sun,
Yunhai Li,
Hao Yu
Affiliations
Yuancheng Peng
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China; Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
Shan Jiang
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Jiayu Wang
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Xuewei Xu
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Ximing Gong
Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore
Weihuan Jin
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Chunxia Song
School of Science, Anhui Agricultural University, Hefei 230036, China
Zhiqiang Dong
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Siyu Sun
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Yunhai Li
State Key Laboratory of Plant Cell and Chromosome, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Hao Yu
Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore; Corresponding author
Summary: Lateral root (LR) initiation is controlled by the pericycle and the neighboring endodermis in Arabidopsis. Here, we demonstrate that UBIQUITIN-SPECIFIC PROTEASE14/DA3 regulates LR initiation by modulating auxin signaling in the pericycle and endodermis. DA3 negatively affects the mRNA and protein levels of AUXIN RESPONSE FACTOR7 (ARF7) and ARF19 in the pericycle and endodermis but positively regulates the protein stability of SHORT HYPOCOTYL 2 (SHY2/IAA3), an auxin signaling repressor, in the endodermis. We show that DA3 interacts with ARF7 and ARF19, inhibiting their binding to the locus of LATERAL ORGAN BOUNDARY DOMAIN16 (LBD16) to repress its expression in the pericycle. SHY2 also interacts with ARF7 and ARF19 in the endodermis and enhances the DA3 repressive effect on ARF7 and ARF19, thus modulating LBD16 expression in the pericycle. Overall, our findings show that DA3 acts with SHY2, ARF7, and ARF19 to coordinate auxin signaling in the pericycle and endodermis to control LR initiation in Arabidopsis.
