Nature Communications (Jul 2019)
Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation
Abstract
The CCR4-NOT complex shortens poly(A) tails of messenger RNAs. By biochemical reconstitution of the entire human CCR4-NOT complex, the authors show the stimulatory roles of non-enzymatic subunits and the importance of the interaction between CAF40 and RNA binding proteins in targeted deadenylation.