Nature Communications (Jul 2019)

Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

  • Tobias Raisch,
  • Chung-Te Chang,
  • Yevgen Levdansky,
  • Sowndarya Muthukumar,
  • Stefan Raunser,
  • Eugene Valkov

DOI
https://doi.org/10.1038/s41467-019-11094-z
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 14

Abstract

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The CCR4-NOT complex shortens poly(A) tails of messenger RNAs. By biochemical reconstitution of the entire human CCR4-NOT complex, the authors show the stimulatory roles of non-enzymatic subunits and the importance of the interaction between CAF40 and RNA binding proteins in targeted deadenylation.