Investigation of Metallo-beta-lactamase Genes (L1 and NDM1) in Isolates of Stenotrophomonas maltophilia

Abstract

Stenotrophomonas maltophilia is resistant to a wide range of antibiotics. This study aimed to investigate the beta-lactamase, ESBL, and MBL enzymes and also L1and NDM1 genes in isolates of Stenotrophomonas maltophilia. Antibiotic susceptibility test, beta-lactamase, ESBL, and MBL test was done on 23 isolated Stenotrophomonas maltophilia. The presence of L1 and NDM1 genes was investigated on isolated MBL bacteria by the PCR method. The results showed the most effective antibiotics were tigecycline and gentamicin (100%) and the highest resistance was observed with aztreonam (56.22%). 39.13% of isolates were ESBL, 82.66% of isolates were beta-lactamase positive, 60.86% of isolates were Metallo-beta-lactamase positive. 39.13% of MBL positive isolates, were positive for the L1 gene but the NDM1 gene was not seen. Results of this study showed the higher resistance to beta-lactam antibiotics also the presence of the l1 gene. ESBL and MBL producing S. maltophilia are frequently resistant to a wide range of antibiotics. Therefore, needs to be done antibiotic sensitivity test, ESBL, and MBL test before treatment for this bacterium.

Keywords

• : Stenotrophomonas maltophilia
• Antibiotic
• L1 metallo-beta-lactamase gene
• New Delhi metallo-beta-lactamase 1 gene