Polycomb-lamina antagonism partitions heterochromatin at the nuclear periphery

Allison P. Siegenfeld; Shelby A. Roseman; Heejin Roh; Nicholas Z. Lue; Corin C. Wagen; Eric Zhou; Sarah E. Johnstone; Martin J. Aryee; Brian B. Liau

doi:10.1038/s41467-022-31857-5

Nature Communications (Jul 2022)

Polycomb-lamina antagonism partitions heterochromatin at the nuclear periphery

Allison P. Siegenfeld,
Shelby A. Roseman,
Heejin Roh,
Nicholas Z. Lue,
Corin C. Wagen,
Eric Zhou,
Sarah E. Johnstone,
Martin J. Aryee,
Brian B. Liau

Affiliations

Allison P. Siegenfeld: Department of Chemistry and Chemical Biology, Harvard University
Shelby A. Roseman: Department of Chemistry and Chemical Biology, Harvard University
Heejin Roh: Department of Chemistry and Chemical Biology, Harvard University
Nicholas Z. Lue: Department of Chemistry and Chemical Biology, Harvard University
Corin C. Wagen: Department of Chemistry and Chemical Biology, Harvard University
Eric Zhou: Department of Chemistry and Chemical Biology, Harvard University
Sarah E. Johnstone: Broad Institute of Harvard and MIT
Martin J. Aryee: Broad Institute of Harvard and MIT
Brian B. Liau: Department of Chemistry and Chemical Biology, Harvard University

DOI: https://doi.org/10.1038/s41467-022-31857-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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Here the authors developed ‘Lamina-Inducible Methylation and Hi-C’ (LIMe-Hi-C) to simultaneously measure chromosome conformation, DNA methylation, and nuclear lamina positioning. Application of the method revealed dynamic changes upon PRC2 inhibition and an essential function of H3K27me3 in regulating sub-compartments and lamina association.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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