Nitrogen addition alleviated sexual differences in responses to cadmium toxicity by regulating the antioxidant system and root characteristics, and inhibiting Cd translocation in mulberry seedlings

Abstract

Cadmium (Cd) toxicity and nitrogen (N) deposition are two major environmental stresses which can affect plant growth. It’s less clear that how the combined Cd accumulation and N deposition affect the male and female plants of dioecious species. The aim of the present study was to detect sex-specific responses to Cd stress and simulated N deposition in one-year-old male, female and hermaphrodite seedlings of Morus alba. Changes in morphology, physiology, root architecture and biomass of the three sex types of mulberry seedlings were determined. The results showed that Cd toxicity caused limited growth, impaired photosynthetic apparatus and decreased gas exchange rates with significant sex-specific differences. Mulberry was found to deploy detoxification mechanisms to avoid or tolerate toxic Cd effects through the activation of the antioxidant system, increasing proline and non-protein thiol contents, translocating Cd into different plant parts and decreasing biomass. Females displayed a low tolerance to high Cd and were more sensitive to Cd stress. Simulated N deposition alleviated the negative effects of Cd on leaves and decreased sex-specific differences in the three kinds of mulberry seedlings, but N fertilizer did not affect the total biomass. The N-stimulated increasing in proline and non-protein thiol contents might play a crucial role in resisting the damage caused by Cd stress, and the three kinds of mulberry seedlings had slightly different ways of improving Cd tolerance by N deposition. Sexual differences in Cd accumulation are correlated with root architecture. This study provides evidence for the utilization of mulberry to treat Cd-contaminated soils under N deposition.

Keywords

• Cadmium
• Mulberry
• Nitrogen deposition
• Sex-specific responses