Overcoming Anxiety Disorder by Probiotic <i>Lactiplantibacillus plantarum LZU-J-TSL6</i> through Regulating Intestinal Homeostasis

Abstract

Lactiplantibacillus plantarum LZU-J-TSL6 with high γ-aminobutyric acid (GABA) production (3.838 g/L) was screened and isolated from the Chinese fermented food snack “Jiangshui”. The improvement effect on anxiety disorder was explored using mice as animal models. In vitro results revealed that LZU-J-TSL6 had the potential to colonize the intestine (p LZU-J-TSL6 was significantly improved (p LZU-J-TSL6 was able to effectively increase the GABA content in the mice hippocampus (p LZU-J-TSL6 increased the relative abundance of beneficial bacteria Bacteroides and Muribaculum, thereby regulating the imbalance of intestinal microbiota caused by anxiety disorder. It also affects the nerve pathway and intestinal mucosal barrier by increasing the content of glutamine and γ-aminobutyric acid and other related metabolites, thereby improving anxiety. Therefore, the GABA-producing Lactobacillus plantus LZU-J-TSL6 can be used as a probiotic to exert an indirect or direct anti-anxiety effect by maintaining the balance of the intestinal environment, producing related metabolites that affect nerve pathways and repair the intestinal mucosal barrier. It can be used as an adjuvant treatment to improve anxiety disorders.

Keywords

• anxiety
• GABA
• gut microbiota
• intestinal mucosal barrier
• <i>Lactiplantibacillus plantarum</i>