Antibacterial Activities and Underlying Mechanisms of the Compound SYAUP-491 against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>
Lina Li,
Yuxin Wang,
He Liu,
Wei Liu,
Xinchen Zhang,
Mengnan An,
Miao Yu,
Yuanhua Wu,
Xinghai Li,
Jianzhong Wang
Affiliations
Lina Li
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Yuxin Wang
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
He Liu
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Wei Liu
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Xinchen Zhang
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Mengnan An
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Miao Yu
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Yuanhua Wu
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Xinghai Li
College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, China
Jianzhong Wang
Institute of Agricultural Quality Standards and Testing Technology, Liaoning Academy of Agricultural Sciences, No. 84 Dongling Road, Shenhe District, Shenyang 110866, China
SYAUP-491 is a novel alkyl sulfonamide. In this study, in vivo and in vitro tests were performed along with a proteomic analysis to determine the effects and underlying mechanisms of the antibacterial activity of SYAUP-491 against the causative agent of bacterial leaf blight in rice. The antibacterial test results suggested that SYAUP-491 exhibited significant activities against Xanthomonas oryzae pv. oryzae (Xoo) in vitro and in vivo. The minimal EC50 values reached 6.96 μg/mL and the curative activity reached 74.1%. Detailed studies demonstrated that SYAUP-491 altered membrane permeability and caused morphological changes. Based on proteomics results, SYAUP-491 might inhibit bacterial protein synthesis. SYAUP-491 may disrupt and alter cell membrane permeability and could further act on ribosomes in the bacterial body. Given the above results, SYAUP-491 could serve as a new lead compound in the research of antibacterial control of plant pathogenic bacterial disease.
