Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and sufficient donors
Vassilis L. Tzounakas,
Anastasios G. Kriebardis,
Hara T. Georgatzakou,
Leontini E. Foudoulaki-Paparizos,
Monika Dzieciatkowska,
Matthew J. Wither,
Travis Nemkov,
Kirk C. Hansen,
Issidora S. Papassideri,
Angelo D׳Alessandro,
Marianna H. Antonelou
Affiliations
Vassilis L. Tzounakas
Department of Biology, Section of Cell Biology and Biophysics, School of Science, NKUA, Athens 15784, Greece
Anastasios G. Kriebardis
Laboratory of Hematology and Transfusion Medicine, Department of Medical Laboratories, Faculty of Health and Caring Professions, Technological and Educational Institute of Athens, Athens 12210, Greece
Hara T. Georgatzakou
Department of Biology, Section of Cell Biology and Biophysics, School of Science, NKUA, Athens 15784, Greece
Leontini E. Foudoulaki-Paparizos
Regional Blood Transfusion Center, “Agios Panteleimon” General Hospital of Nikea, Piraeus 18454, Greece
Monika Dzieciatkowska
Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine–Anschutz Medical Campus, Aurora, 80045 CO, USA
Matthew J. Wither
Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine–Anschutz Medical Campus, Aurora, 80045 CO, USA
Travis Nemkov
Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine–Anschutz Medical Campus, Aurora, 80045 CO, USA
Kirk C. Hansen
Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine–Anschutz Medical Campus, Aurora, 80045 CO, USA
Issidora S. Papassideri
Department of Biology, Section of Cell Biology and Biophysics, School of Science, NKUA, Athens 15784, Greece
Angelo D׳Alessandro
Department of Biochemistry and Molecular Genetics, University of Colorado, School of Medicine–Anschutz Medical Campus, Aurora, 80045 CO, USA; Correspondence to: Dept. of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Denver, 12801 East 17th Ave, Rm L18-9403, Aurora, CO 80045, USA.
Marianna H. Antonelou
Department of Biology, Section of Cell Biology and Biophysics, School of Science, NKUA, Athens 15784, Greece; Corresponding author. Fax: +30 210 727 4742.
This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage in standard blood bank conditions compared to control, G6PD sufficient (G6PD+) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in RBCs throughout the storage period, with or without stimulation by oxidants, supplementation of N-acetylcysteine and energy depletion, following incubation of stored cells for 24 h at 37 °C. Apart from cell characteristics, the total or uric acid-dependent antioxidant capacity of the supernatant in addition to extracellular potassium concentration was determined in RBC units. Finally, procoagulant activity and protein carbonylation levels were measured in the microparticles population. Further information can be found in “Glucose 6-phosphate dehydrogenase deficient subjects may be better “storers” than donors of red blood cells” [1]. Keywords: G6PD deficiency, Red blood cell storage lesion, Oxidative stress, Cell fragility, Microparticles
