Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

Abstract

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

Keywords

• Plasmodium
• malaria
• telomerase
• assay
• nonradioactive