Construction of a Secondary Enclosure for UVB Irradiation of Mice

Justin Choi; Zachary A. Bordeaux; Gabriella Braun; Cole Davis; Varsha Parthasarathy; Junwen Deng; Mathew T. Taylor; Anusha Kambala; Hannah Cornman; Olusola Oladipo; Martin P. Alphonse; Cameron E. West; Shawn G. Kwatra; Madan M. Kwatra

JID Innovations (Jan 2023)

Construction of a Secondary Enclosure for UVB Irradiation of Mice

Justin Choi,
Zachary A. Bordeaux,
Gabriella Braun,
Cole Davis,
Varsha Parthasarathy,
Junwen Deng,
Mathew T. Taylor,
Anusha Kambala,
Hannah Cornman,
Olusola Oladipo,
Martin P. Alphonse,
Cameron E. West,
Shawn G. Kwatra,
Madan M. Kwatra

Affiliations

Justin Choi: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA
Zachary A. Bordeaux: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA
Gabriella Braun: Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA
Cole Davis: Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA
Varsha Parthasarathy: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Junwen Deng: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Mathew T. Taylor: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Anusha Kambala: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Hannah Cornman: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Olusola Oladipo: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Martin P. Alphonse: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Cameron E. West: Genzada Pharmaceuticals, Hutchinson, Kansas, USA
Shawn G. Kwatra: Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Correspondence: Shawn G. Kwatra, Department of Dermatology, Johns Hopkins University School of Medicine, Suite 206, Cancer Research Building II, 1550 Orleans Street, Baltimore, Maryland 21231, USA.
Madan M. Kwatra: Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA; Department of Pharmacology & Cancer Biology, Duke University School of Medicine, Durham, North Carolina, USA

Journal volume & issue: Vol. 3, no. 1
p. 100164

Abstract

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UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.

Published in JID Innovations

ISSN: 2667-0267 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Dermatology
Website: https://www.journals.elsevier.com/jid-innovations

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