Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina
Tomasz Kulik,
Maciej Buśko,
Katarzyna Bilska,
Anna Ostrowska-Kołodziejczak,
Anne D. van Diepeningen,
Juliusz Perkowski,
Sebastian Stenglein
Affiliations
Tomasz Kulik
Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, Poland
Maciej Buśko
Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland
Katarzyna Bilska
Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn 10-727, Poland
Anna Ostrowska-Kołodziejczak
Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland
Anne D. van Diepeningen
CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands
Juliusz Perkowski
Department of Chemistry, Poznań University of Life Sciences, ul. Wojska Polskiego 75, Poznań 60-625, Poland
Sebastian Stenglein
Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-Comisión de Investigaciones Científicas de la provincia de Buenos Aires-Instituto de Investigaciones en Biodiversidad y Biotecnología-Consejo Nacional de Investigaciones Científicas y Técnicas (CICBA-INBIOTEC, CONICET), Av. República de Italia 780, Azul 7300, Buenos Aires, Argentina
Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.
