<i>Rehmannia glutinosa</i> Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
Heng Ren,
Kejie Li,
Yan Min,
Binhang Qiu,
Xiaolu Huang,
Jingxin Luo,
Liwen Qi,
Maoli Kang,
Peng Xia,
Hanzhen Qiao,
Jun Chen,
Yaoming Cui,
Liping Gan,
Peng Wang,
Jinrong Wang
Affiliations
Heng Ren
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Kejie Li
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Yan Min
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Binhang Qiu
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Xiaolu Huang
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Jingxin Luo
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Liwen Qi
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Maoli Kang
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Peng Xia
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Hanzhen Qiao
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Jun Chen
Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Province Key Innovation Center of Integration in Production and Education for High-Quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang 330045, China
Yaoming Cui
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Liping Gan
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Peng Wang
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Jinrong Wang
School of Bioengineering, Henan University of Technology, Lianhua Street, Hi-Tech Zone, Zhengzhou 450000, China
Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of Rehmannia glutinosa polysaccharides (RGP) is optimized using two methods—the AB-8 macroporous resin (RGP-1) method and the H2O2 (RGP-2) method. The optimal decolorization parameters for the AB-8 macroporous resin method were as follows: temperature, 50 °C; macroporous resin addition, 8.4%; decolorization duration, 64 min; and pH, 5. Under these conditions, the overall score was 65.29 ± 3.4%. The optimal decolorization conditions for the H2O2 method were as follows: temperature, 51 °C; H2O2 addition, 9.5%; decolorization duration, 2 h; and pH, 8.6. Under these conditions, the overall score was 79.29 ± 4.8%. Two pure polysaccharides (RGP-1-A and RGP-2-A) were isolated from RGP-1 and RGP-2. Subsequently, their antioxidant and anti-inflammatory effects and mechanisms were evaluated. RGP treatment activated the Nrf2/Keap1 pathway and significantly increased the activity of antioxidant enzymes (p p < 0.05). RGP-1-A had a significantly better protective effect than RGP-2-A, likely owing to the sulfate and uronic groups it contains. Together, the findings indicate that RGP can act as a natural agent for the prevention of oxidation and inflammation-related diseases.
